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PKA (RIIβ) (pS114) Mouse anti-Human, Alexa Fluor 488, Clone: 47, BD

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BDB560204 50 Tests
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Catalog No. BDB560204 Supplier BD Biosciences Supplier No. 560204

Description

Mouse Monoclonal Antibody

cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ). Most cells, including T lymphocytes, express both type I and type II PKAs. RIIa expression is associated with cellular transformation, while RIIβ expression correlates with mitotic arrest and cellular differentiation. Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The cAMP-dependent autophosphorylation of the human RIIβ subunits occurs at serine 114 (S114). In addition to their enzyme regulatory activity, the RIIα and RIIβ subunits determine the subcellular location of the holoenzymes via their interactions with specific intracellular anchoring proteins. The 47/PKA monoclonal antibody recognizes the phosphorylated S114 in the RIIβ subunit of PKA. The orthologous phosphorylation site in mouse and rat PKA(RIIβ) is S112.

Host Species: Mouse
Clone: 47
Isotype: IgG1 κ
Species Reactivity: Human
Immunogen: Phosphorylated Human PKA(RIIβ) Peptide

Intracellular Staining

TRUSTED_SUSTAINABILITY

Specifications

Antigen PKA (RIIβ) (pS114)
Applications Flow Cytometry
Classification Monoclonal
Clone 47
Conjugate Alexa Fluor 488
Description KAP3, PKAR2B, PRKAR2B
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Phosphorylated Human PKA(RIIβ) Peptide
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Research Discipline Cell Biology
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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