PKA RIIα (pS99) Mouse anti-Human, Alexa Fluor 647, Clone: I65-856.286, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 560164

Catalog No. BDB560164

Description

cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ). Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The cAMP-dependent autophosphorylation of the human RIIα subunit occurs at Serine 99 (S99) (Entrez Protein Accession No.CAA33094). Most cells express both type I and type II PKAs. Although the Rα isoforms are ubiquitously expressed, the Rβ isoforms are predominantly found in nervous and adipose tissues. In addition to their enzyme regulatory activity, the RIIα and RIIβ subunits determine the subcellular location of the holoenzymes via their interactions with specific intracellular anchoring proteins. The I65-856.286 antibody recognizes human PKA RIIα phosphorylated at S99.

Host Species: Mouse
Clone: I65-856.286
Isotype: IgG1 κ
Species Reactivity: Human
Immunogen: Phosphorylated Human PKA RIIα Peptide

Intracellular Staining

Specifications

• Antigen: PKA RIIα (pS99)
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: I65-856.286
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human PKA RIIα Peptide
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov