PKCα Mouse anti-Human, Alexa Fluor 488, Clone: 3/PKC, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 560242

Catalog No. BDB560242

Description

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. Conventional PKC (cPKC) subfamily members (α, β, and γ isoforms) consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca²⁺, phospholipid, diacylglycerol, or phorbol ester. However, the the C2-like domains of
novel PKC (nPKC) subfamily members (δ, ε, η, and θ isoforms) are Ca²⁺-independent. The atypical PKC (aPKC) subfamily members (ζ, ι,
and λ isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCα regulates a wide variety of functions such as cellular growth, apoptosis, cardiomyocyte function, and brain cognitive functions. The 3/PKCα monoclonal antibody recognizes PKCα, regardless of phosphorylation status, and has been reported to crossreact with PKCβ.

Host Species: Mouse
Clone: 3
Isotype: IgG2b, κ
Species Reactivity: Human
Immunogen: Human PKCα aa. 270-427 Recombinant Protein

Intracellular Staining

Specifications

• Antigen: PKCα
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Human PKCα aa. 270-427 Recombinant Protein
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG2b κ

• Applications: Flow Cytometry
• Clone: 3/PKC
• Description: Protein Kinase C α, PKC-α, PKC-A, PKCA, PRKCA
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.