MilliporeSigma™ anti-PKCzeta Clone: 4B12.3,

Catalog No.	Quantity	Conjugate
MABC946MI	100 μg	Unconjugated

Catalog No. MABC946MI Supplier MilliporeSigma™ Supplier No. MABC946

Description

Mouse Monoclonal Antibody

Specifically detects PKCζ Clone: 4B12.3 in Human, Mouse, Rat samples, and it is validated for Immunohistochemistry (Paraffin), Western Blotting

Protein kinase C zeta type (EC 2.7.11.13; UniProt Q05513; also known as nPKC-zeta, PKC-zeta) is encoded by the PRKCZ (also known as PKC2) gene (Gene ID 5590) in human. Protein kinase Cs (PKCs) are threonine/serine kinases that play important roles in cellular signal transductions. PKC isozymes are classified into three major classes based on their domain compositions and activator requirements. The conventional PKCs (cPKCs) include the alpha, beta I, beta II, and gamma isoforms. The novel PKCs (nPKCs) include the delta, epsilon, eta, and theta isoforms. Atypical PKCs (aPKCs) include the lambda (mouse ortholog to human PKC-lambda is called PKC-iota) and zeta isoforms. Both cPKCs and nPKCs bind diacylglycerol (DAG) via their C1 domain, while only cPKCs, but not nPKCs bind Ca+2 via their C2 domain. The aPKCs do not bind either DAG or calcium for activity. All three classes of PKCs contain a pseudosubstrate sequence in their N-terminal region that keeps the substrate-binding cavity occupied via intramolecular interaction when the enzymes are in their inactive conformation. Conformation change upon enzyme activation frees the substrate-binding site.

Specifications

• Antigen: PKCζ
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 4B12.3
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: Q05513
• Gene Symbols: PRKCZ, PKC2
• Host Species: Mouse
• Immunogen: GST-tagged recombinant human PKCζ C-terminal fragment.
• Purification Method: Protein G purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Cancer
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_002735
• Test Specificity: Expected to react with all three spliced isoforms of human PKC-zeta.
• Target Species: Human, Mouse, Rat
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only