PKR Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1990
PA1-990 detects PKR in human and mouse samples. PA1-990 has been successfully used in Western blot and ICC/IF procedures. In Western blot analysis of HeLa cell cytosol this antibody detects a ~66 kDa protein representing PKR. The PA1-990 immunogen is a synthetic peptide corresponding to residues L(435) V T S L K N D G K R T R S K (449) of human PKR.PKR is a 68 kDa protein and an IFN-induced serine/threonine protein kinase consisting of two N-terminal RNA-binding regulatory domains and a functional C-terminal serine/threonine protein kinase domain. Activated by dsRNA produced during viral infections, PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. Upon activation, PKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & maligcy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
|Synthetic peptide corresponding to residues L(435) V T S L K N D G K R T R S K (449) of human PKR.|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Protein Kinase R, eIF2a Kinase, ds RNA-Dependent Protein Kinase|
|-20° C, Avoid Freeze/Thaw Cycles|
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