PLC-γ Mouse anti-Human, Alexa Fluor 488, Clone: 10/PLCGAMMA, BD

Catalog No.	Quantity
BDB558566	50 Tests

Catalog No. BDB558566 Supplier BD Biosciences Supplier No. 558566

Description

Mouse Monoclonal Antibody

The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCγ is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCγ1, which is ubiquitously expressed, and 2) PLCγ2, found primarily in the lymphoid system. PLCγ is essential for growth factor-induced cell motility and mitogenesis. PLCγ1 null mice exhibit retarded embryonic growth and lethality in midgestation. Overexpression of PLCγ is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLCγ activity by growth factors is involved in cell growth and transformation. The 10/PLCgamma monoclonal antibody recognizes PLCγ1, regardless of phosphorylation status. It does not cross-react with PLCγ2.

Intracellular Staining

Specifications

• Antigen: PLC-γ
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 10/PLCGAMMA
• Conjugate: Alexa Fluor 488
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Cow PLCγ1 N-terminal region Peptide
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1