PLK1 (pT210) Mouse, Alexa Fluor 647, Clone: K50-483, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 558447

Catalog No. BDB558447

Description

Polo-like kinase (PLK1) is a serine/threonine kinase with structural similarities to Drosophila's Polo kinase and the Cdc5p of Saccharomyces cerevisiae. Like its invertebrate counterparts, PLK1 activity is required for DNA synthesis and is regulated throughout the cell cycle. Furthermore, PLK1 is highly expressed in primary tumors. It associates with the mitotic spindle during mitosis suggesting that, in addition to its role during S phase, PLK1 may play a role during chromosome segregation. This is consistent with its potential role in cancer development. Threonine 210 (T210) is one of the major phoshorylation sites in activated PLK1 obtained from human mitotic cells. The K50-483 monoclonal antibody recognizes the phosphorylated T210 of human PLK1.

Intracellular Staining

Specifications

• Antigen: PLK1 (pT210)
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: K50-483
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human PLK1 Peptide
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Human, Mouse
• Isotype: IgG1 κ