Invitrogen™ pro-Caspase 3 Monoclonal Antibody (31A893)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA141163

Catalog No. 01-676-479

Description

Suggested positive control: HL-60 or staurosporine treated apoptotic HeLa.

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Specifications

• Antigen: pro-Caspase 3
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P42574
• Gene Symbols: CASP3
• Immunogen: Recombinant full-length human Caspase-3 protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 31A893
• Conjugate: Unconjugated
• Gene: CASP3
• Gene Alias: A830040C14Rik; AC-3; Apopain; Casp3; CASP-3; caspase 3; caspase 3, apoptosis related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; CC3; Cpp32; CPP-32; CPP32B; cysteine protease CPP32; LICE; mldy; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1; Yama
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 836
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid