PSME1, Rabbit, Monoclonal Antibody, Abnova™

Rabbit monoclonal antibody raised against a human PSME1 peptide using ARM Technology.

Supplier:  Abnova Corporation H00005720K

Catalog No. 89-210-239

Includes: Up to three rabbit IgG clones of 1mg each will be delivered to customer.

Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 nonidentical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a nonlysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified. [provided by RefSeq

Library Construction: Nonfusion antibody library from rabbit spleen (ARM Technology)
Expression: Overexpression vector and transfection into 293H cell line

Order Info

1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.

Specifications

• Antigen: PSME1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: In 1X PBS, pH 7.4
• Gene Accession No.: PSME1
• Gene Symbols: PSME1
• Immunogen: A synthetic peptide of human PSME1
• Regulatory Status: RUO
• Gene ID (Entrez): 5720
• Content And Storage: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG

• Applications: ELISA, Western Blot
• Concentration: 1mg
• Description: proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
• Gene: PSME1
• Gene Alias: IFI5111, MGC8628, PA28A, PA28alpha, REGalpha
• Host Species: Rabbit
• Quantity: 1 mg x up to 3 Abs
• Primary or Secondary: Primary
• Target Species: Human
• Form: Liquid