MilliporeSigma™ anti-PTPRT Clone: 1F7,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1158

Catalog No. MABS1158MI

Description

Specifically detects PTPRT Clone: 1F7 in Human, Mouse, Rat samples, and it is validated for Western Blotting

Receptor-type tyrosine-protein phosphatase T (EC 3.1.3.48; UniProt O14522; also known as R-PTP-T, Receptor protein tyrosine phosphatase, Receptor-type tyrosine-protein phosphatase rho, RPTP-rho, RPTPrho) is encoded by the PTPRT (also known as KIAA0283) gene (Gene ID 11122) in human. RPTPrho/PTPRT is a member of the R2B subfamily of receptor-type protein tyrosine phosphatases (R-PTPs). Known RPTPrho/PTPRT substrates include BCR (Tyr177), paxillin (Tyr188), and STAT3 (Tyr705). In addition, in vitro pull-downs and cell-free dephosphorylation assays also suggest cadherin and catenin family of cell adhesion molecules as physiological substrates of RPTPrho/PTPRT. PTPRT is the single most commonly mutated PTPR gene in all sequenced human cancers, with the highest PTPRT mutation frequency found in cutaneous melanoma. Among 16 tumor types examined, 37.9% of PTPRT mutations are found in the catalytic (PTPase) domain and 33.0% in the extracellular fibronectin type III-like (FN3) domain. In head and neck squamous cell carcinoma (HNSCC), 45.5% PTPRT mutations are located in the PTPase domain, leading to up-regulated STAT3 phosphorylation in HNSCC tumors.

Specifications

• Antigen: PTPRT
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: O14522
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 1F7 reacts with the full-length PTPRT as well as cleaved/truncated PTPRT forms that contain the two phosphatase domains.
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG1 κ

• Applications: Western Blot
• Clone: 1F7
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: PTPRT, KIAA0283
• Immunogen: GST-tagged recombinant protein corresponding to the PTPase domains 1/2 of human PTPRT.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_008981
• Target Species: Human, Mouse, Rat
• Form: Purified

For Research Use Only