PU.1 Mouse anti-Human, Unlabeled, Clone: G148-74, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 554268

Catalog No. BDB554268

Description

PU.1 is an ets-related transcription factor expressed in B lymphocytes and macrophages. It was initially identified as the oncogene Spi-1 which was found to block erythroblast differentiation. PU.1 functions by binding transcriptional control elements such as μB in the immunoglobulin heavy chain enhancer and π adjacent to μE2. It has been shown to form a complex with NF-EM5, a B cell transcription factor, resulting in the activation of the immunoglobulin κ 3' enhancer. PU.1 also appears to be critically involved in the control of monocyte development by regulating the expression of the macrophage colony-stimulating factor receptor. PU.1 runs as a 40-42kDa protein on SDS-PAGE. G148-74 recognizes human PU.1, an Ets-related transcription factor. PU.1 is expressed in B lymphocytes and macrophages and like other Ets-related proteins, binds to consensus sites in DNA through an 85 amino acid Ets domain in the carboxyl terminal region of the protein. The antibody was raised against a bacterially expressed GST-PU.1 fusion protein.

Host Species: Mouse
Clone: G148-74
Isotype: IgG2a
Species Reactivity: Human
Immunogen: PU.1 Fusion Protein
Formula Weight [Chemical]: 40-42kDa

Immunoprecipitation, Western Blotting

Specifications

• Antigen: PU.1
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: PU.1 Fusion Protein
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG2a

• Applications: Western Blot
• Clone: G148-74
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.