Invitrogen™ PYGL Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA533012

Catalog No. PIPA533012

Description

Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Dog (100%); Gibbon, Marmoset, Panda (90%); Mouse, Sheep, Hamster, Horse, Rabbit (80%). For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

Specifications

• Antigen: PYGL
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PYGL
• Gene Alias: glycogen liver; Glycogen phosphorylase, liver form; GPLL; GSD6; Lgp; liver glycogen phosphorylase; phosphorylase, glycogen, liver; PYGL
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 403738, 5836
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: P06737
• Gene Symbols: PYGL
• Immunogen: Synthetic 10 amino acid peptide from internal region of human PYGL.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Canine
• Product Type: Antibody
• Isotype: IgG