Rab5 Mouse, Unlabeled, Clone: 1, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 610724

Catalog No. BDB610724

Description

Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post translationally modified by geranylgeranylation which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 regulates early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 may insert into membranes by a multistep process in a which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: Rab5
• Clone: 1
• Conjugate: Unconjugated
• Format: Affinity Purified
• Immunogen: Human Rab5 aa. 1-215
• Quantity: 50μg
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Canine, Human

• Applications: Western Blot
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Host Species: Mouse
• Isotype: IgG_2a
• Regulatory Status: RUO
• Storage Requirements: Store undiluted at -20°C.
• Monoclonal or Polyclonal: Monoclonal

For Research Use Only.