MilliporeSigma™ anti-Rac1 Alexa Fluor 647, Clone: 23A8,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 05389AF647

Catalog No. 05-389-AF647

Description

Specifically detects Rac1 Clone: 23A8 in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry

Ras-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.

Specifications

• Antigen: Rac1
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Gene Accession No.: P63000
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG2b

• Applications: Immunocytochemistry
• Clone: 23A8
• Formulation: Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1% sodium azide.
• Gene Symbols: RAC1, TC25, MIG5
• Immunogen: Recombinant protein containing the full length human Rac.
• Quantity: 100 μL
• Research Discipline: Signaling
• Gene ID (Entrez): NM_006908
• Target Species: Human, Mouse, Rat
• Form: Purified

For Research Use Only