RAD51 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA14985

Catalog No. PIPA14985

Description

PA1-4985 detects Rad51 from human, mouse, and chicken samples. PA1-4985 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and indirect immunofluorescence applications. By Western blot, this antibody detects a 37 kDa band representing Rad51 from Hela cells. GFP-tagging of Rad51 protein at either N- or C- terminus inactivates the function of Rad51 and therefore it cannot be used for the study of foci formation instead of the antibody. The PA1-4985 immunogen is full-length recombinant Rap51 protein.

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

Specifications

• Antigen: RAD51
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Rad51
• Gene Alias: AV304093; BRCA1/BRCA2-containing complex, subunit 5; BRCC5; DNA repair protein RAD51; DNA repair protein RAD51 homolog 1; DNA repair protein RAD51 homolog A; DNA repair protein rhp51; FANCR; homolog to S.cerevisiae; HRAD51; HsRad51; HsT16930; MGC128559 protein; MRMV2; MUT5; rad51; RAD51 homolog; RAD51 homolog (RecA homolog); RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RAD51 recombinase; RAD51 recombinase L homeolog; rad51.1; rad51.L; rad51-1; RAD51A; rad51-a; rad51-b; Reca; RecA family recombinase Rhp51; RecA, E. coli, homolog of; RecA-like protein; recombinase RAD51; recombination protein A; RGD1563603; rhp51; SPAC644.14c; wu:fb38e12; xrad51; XRad51.1; YER095W; zgc:77754
• Host Species: Rabbit
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Chicken, Human, Mouse, X. laevis
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Concentration: Conc. Not Determined
• Formulation: whole serum with 0.09% sodium azide
• Gene Accession No.: P37383, Q06609, Q08297
• Gene Symbols: Rad51
• Immunogen: Purified full-length recombinant Rad51 protein.
• Regulatory Status: RUO
• Gene ID (Entrez): 19361, 396086, 5888
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov