Ig Goat anti-Rat, Brilliant Violet 480, Polyclonal, BD
Goat Polyclonal Antibody
Manufacturer: BD Biosciences 564878
BD Horizon BV480 Goat Anti-Rat Ig is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rat Ig primary antibodies. It also stains rat B cells and has little reactivity with rat non-B cells or mouse splenocytes. As a second step, it is reactive with rat IgG and IgM monoclonal antibodies although a weaker signal may be detected when the primary antibody has a rat IgM or IgG2b isotype due to its adsorption with mouse Ig. It has weak cross-reactivity detectable by flow cytometry with some, but not all, hamster immunoglobulins.
The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant Violet family of dyes. This dye has been exclusively developed by BD Biosciences as an optimal dye for imaging applications. BD Horizon BV480 has an Ex Max at 436nm and Em Max at 477 nm. The use of a mounting reagent (eg, ProLong Gold) is highly recommended to maximize the photostability of BV480.
|Brilliant Violet 480|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|The Polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.|
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