MilliporeSigma™ anti-RNA polymerase II Alexa Fluor 647, Clone: CTD4H8,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 05623AF647

Catalog No. 05-623-AF647

Description

Specifically detects RNA polymerase II Clone: CTD4H8 in Human, Mouse samples, and it is validated for Immunocytochemistry

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Specifications

• Antigen: RNA polymerase II
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Gene Accession No.: P24928
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes Phospho- and non-phospho-RNA polymerase II.
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG1

• Applications: Immunocytochemistry
• Clone: CTD4H8
• Formulation: Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1% sodium azide.
• Gene Symbols: POLR2A, POLR2
• Immunogen: KLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
• Quantity: 100 μL
• Research Discipline: Epigenetics, Nuclear Function
• Gene ID (Entrez): NP_000928.1
• Target Species: Human, Mouse
• Form: Purified

For Research Use Only