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RORγt Mouse anti-Mouse, Brilliant Violet 421, Clone: Q31-378, BD

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Description

Mouse Monoclonal Antibody

The Q31-378 monoclonal antibody recognizes mouse RORgamma t (RORγt), an isoform of RORgamma (RORγ). RORγt is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. RORγt is expressed exclusively by lymphoid cells including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells, NKT cells and innate lymphoid cells such as lymphoid tissue inducer (LTi) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches.
The antibody was conjugated to Brilliant Violet™ 421 and has been developed in collaboration with Sirigen. With an Ex Max of 407nm and Em Max at 421nm, Brilliant Violet™ 421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). Brilliant Violet™ 421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

Host Species: Mouse
Clone: Q31-378
Isotype: IgG2a, κ
Species Reactivity: Ms
Immunogen: Mouse RORγt Recombinant Protein

Flow Cytometry, Intracellular Staining

TRUSTED_SUSTAINABILITY

Specifications

Antigen RORγt
Applications Flow Cytometry
Classification Monoclonal
Clone Q31-378
Concentration 0.2mg/mL
Conjugate Brilliant Violet 421
Description RORγT; RORgt; RORgamma t; RORgammaT; Rorc2; Rorg; TOR; Thor; Nr1f3
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Mouse RORγt Recombinant Protein
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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