RORγt Mouse anti-Mouse, Brilliant Violet 421, Clone: Q31-378, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 562894

Catalog No. BDB562894

Description

The Q31-378 monoclonal antibody recognizes mouse RORgamma t (RORγt), an isoform of RORgamma (RORγ). RORγt is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. RORγt is expressed exclusively by lymphoid cells including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells, NKT cells and innate lymphoid cells such as lymphoid tissue inducer (LTi) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches.
The antibody was conjugated to Brilliant Violet™ 421 and has been developed in collaboration with Sirigen. With an Ex Max of 407nm and Em Max at 421nm, Brilliant Violet™ 421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). Brilliant Violet™ 421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

Host Species: Mouse
Clone: Q31-378
Isotype: IgG2a, κ
Species Reactivity: Ms
Immunogen: Mouse RORγt Recombinant Protein

Flow Cytometry, Intracellular Staining

Specifications

• Antigen: RORγt
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: RORγT; RORgt; RORgamma t; RORgammaT; Rorc2; Rorg; TOR; Thor; Nr1f3
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: Q31-378
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Mouse RORγt Recombinant Protein
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov