MilliporeSigma™ anti-ROR2 Clone: Nt2535-2835,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1152

Catalog No. MABS1152MI

Description

Specifically detects ROR2 Clone: Nt2535-2835 in Human, Mouse samples, and it is validated for ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blotting

Tyrosine-protein kinase transmembrane receptor ROR2 (EC 2.7.10.1; UniProt Q9Z138; also known as mRor2, Ntrkr2, Neurotrophic tyrosine kinase receptor-related 2) is encoded by the Ror2 gene (Gene ID 26564) in murine species. Ror2 is a receptor tyrosine kinase that binds Wnt5a and negatively regulates canonical Wnt signaling. Although in C. elegans and Xenopus Ror2 is reported to modulate Wnt signaling nonautonomously by preventing Wnt from stimulating adjacent cells, studies conducted in mammalian cells suggest that Ror2 cytoplasmic domain is crucial for its function and Ror2 inhibits Wnt signaling in a cell-autonomous manner. Ror2 and the closely related Ror1 play essential roles during development. Human ROR2 gene mutations have been associated with dominant Brachydactyly B and recessive Robinow syndrome. Ror2-null mice exhibit skeletal abnormalities similar to the human syndromes. In addition, these mice display heart and lung defects and die perinatally. Ror2 is initially translated with an N-terminal signal sequence (a.a. 1-33), the removal of which yields the mature receptor with an extracellular region (a.a. 34-403), a transmembrane segment (a.a. 404-424), followed by the cytoplasmic region (a.a. 425-944) that contains the kinase domain (a.a. 473-746), a Pro-rich domain (a.a. 784-857) sandwiched between two Ser/Thr-rich domains (a.a. 753-782 and a.a. 859-882).

Specifications

• Antigen: ROR2
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: Q9Z138
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Form: Purified

• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
• Clone: Nt2535-2835
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: Ror2
• Immunogen: Recombinant protein corresponding to the second Ser/Thr-rich domain of mouse mRor2.
• Quantity: 100 μg
• Research Discipline: Signaling
• Test Specificity: Clone Nt 2535-2835 is specific to the mouse and human ROR2, but does not detect mouse ROR1. Clone Nt 2535-2835 exclusively detects ROR2 protein in paraffin-embedded tissues (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG1 κ

For Research Use Only