SLP-76 (pY113) Mouse anti-Human, Unlabeled, Clone: J80-373, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 558388

Catalog No. BDB558388

Description

SLP-76 (SH2 domain-containing Leukocyte Protein of 76kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR) -mediated intracellular signaling pathway. It may be involved in the signaling pathways of other peripheral blood leukocytes; thymic/splenic cells; and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Gads, Grb2, PLCg1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes. An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors. The phosphorylated tyrosine 113 (Y113) brings into the signaling complex the Rho-family guanine-nucleotide exchange factor Vav1, which is involved in the formation of a multimolecular assembly that participates in TCR-stimulated actin cytoskeletal rearrangement. The J80-373 monoclonal antibody recognizes the phosphorylated Y113 of activated SLP-76.

Western Blotting

Specifications

• Antigen: SLP-76 (pY113)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human SLP-76 Peptide
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Western Blot
• Clone: J80-373
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov