Smad1 (pS463/pS465) Rat, Unlabeled, Clone: N6-1233, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 562508

Catalog No. BDB562508

Description

The N6-1233 monoclonal antibody specifically binds to the Smad1 protein phosphorylated at the Ser463/465 sites and the Smad8 protein phosphorylated at the Ser465/467 sites. Smad1 and Smad8 are ∽60 kDa proteins and are members of the Smad superfamily. The Smad family members are divided into three subfamilies: receptor regulated Smads or R-Smads, including Smads1, 2, 3, 5, and 8; common partner Smad, or Co-Smad, including Smad4; and inhibitory Smads, or I-Smad, including Smads 6 and 7. Activation of Transforming Growth Factor-beta (TGF-beta) superfamily serine/threonine kinase receptors, such as TGF-beta and Bone MorphogenicProtein (BMP) receptors, leads to the phosphorylation of R-Smads at several sites. It has been shown that Ser463 and Ser465 of Smad1 are phosphorylated by BMP receptors. In B cells and pre-B cells, BMP-6 has been shown to induce Smad1/5/8 phosphorylation and inhibit cell proliferation. Phosphorylated R-Smads form complexes with Co-Smad and translocate into the nucleus to regulate transcription affecting a wide range of critical processes including cell-fate determination, proliferation, morphogenesis, differentiation and apoptosis. The inhibitory Smads inhibit this pathway through two potential mechanisms: either by preventing R-Smads from binding to their corresponding receptors and/or by competing with Smad4, the Co-Smad, from binding to R-Smads. This antibody may crossreact with Smad5 pS463/pS465 based on sequence homology.

Specifications

• Antigen: Smad1 (pS463/pS465)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: Smad1,Smad8/Smad9; MADH1, MADH9
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Isotype: IgG2a κ

• Applications: Western Blot
• Clone: N6-1233
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human Smad1 aa 456-465 Peptide
• Quantity: 0.1 mg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C