SPAM1, Rabbit, Monoclonal Antibody, Abnova™

Rabbit monoclonal antibody raised against a human SPAM1 peptide using ARM Technology.

Supplier:  Abnova Corporation H00006677K

Catalog No. 89-200-111

Includes: Up to three rabbit IgG clones of 1mg each will be delivered to customer.

Description

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq

Library Construction: Nonfusion antibody library from rabbit spleen (ARM Technology)
Expression: Overexpression vector and transfection into 293H cell line

Order Info

1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.

Specifications

• Antigen: SPAM1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: In 1X PBS, pH 7.4
• Gene Accession No.: SPAM1
• Gene Symbols: SPAM1
• Immunogen: A synthetic peptide of human SPAM1
• Regulatory Status: RUO
• Gene ID (Entrez): 6677
• Content And Storage: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG

• Applications: ELISA, Western Blot
• Concentration: 1mg
• Description: sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
• Gene: SPAM1
• Gene Alias: HYA1, HYAL1, HYAL3, HYAL5, MGC26532, PH-20, PH20, SPAG15
• Host Species: Rabbit
• Quantity: 1 mg x up to 3 Abs
• Primary or Secondary: Primary
• Target Species: Human
• Form: Liquid