α-Spectrin II Mouse, Unlabeled, Clone: 35, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB612560	BD Biosciences 612560	50 μg
BDB612561	BD Biosciences 612561	150 μg

Description

Spectrins are central components of the cytoskeleton that form a scaffold below the plasma membrane. Spectrins contain two subunits, α and β, which intertwine to form heterodimers that can self associate into elongated tetramers. α-spectin I and β-spectrin I form heterodimers in red blood cells, while nonerythroid mammalian cells contain heterodimers of α-spectin I and II with β-spectrin I to V. The structure of spectrins includes a succession of triple-helical repeats along with various domains, such as SH3 domain, EF hands, PH domains, and binding domains for ankyrin, actin, band 4.1, and calmodulin. α-spectrin II is a widely expressed non-erythroid α-spectrin that contains an SH3 domain, a calmodulin binding site, and two cleavage sites for proteases, such as calpains and caspase-3. β-spectrin II is a widely expressed non-erythroid β-spectrin that contains a C-terminal region that interacts with α-spectrins and a PH domain. α-spectrin II and β-spectrin II, like many other spectrins, can form heterodimers that can self associate into tetramers, as well as interact with Band 4.1, F-actin, and other proteins near the plasma membrane. This scaffold of cytoskeletal and plasma membrane proteins is critical for the maintenance of cell structure.

Immunofluorescence, Western Blotting

Specifications

• Antigen: α-Spectrin II
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG1
• Purification Method: Affinity Purified

• Clone: 35
• Applications: Immunofluorescence, Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Canine, Chicken, Human
• Immunogen: Human α-Spectrin II aa. 252-371
• Primary or Secondary: Primary

For Research Use Only.