SSEA-4 Mouse, PE-CF594, Clone: MC813-70, BD

Catalog No.	Quantity
BDB562487	50 Tests

Catalog No. BDB562487 Supplier BD Biosciences Supplier No. 562487

Description

Mouse Monoclonal Antibody

The MC813-70 monoclonal antibody reacts with Stage-Specific Embryonic Antigen-4 (SSEA-4), a carbohydrate epitope on the major ganglioside, but not the neutral glycolipid, of human teratocarcinoma cells. As its name implies, the expression of SSEA-4 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern differs in the human and mouse. In the human, SSEA-4 is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (ICM), embryonic stem (ES) cells, and the K562 erythromyeloid leukeumia cell line. As human stem cells undergo differentiation, SSEA-4 expression is lost. In the mouse, SSEA-4 is found on oocytes and early cleavage-stage embryos, and primitive ectoderm, but not on EC, ICM, or ES cells. In some cases, SSEA-4 expression appears upon differentiation of mouse EC or ES cells. This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488nm), green (532nm) and yellow-green (561nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20nm filter).

Host Species: Mouse
Clone: MC813-70
Isotype: IgG3
Species Reactivity: Human
Immunogen: Human Teratocarcinoma Cell Line

Flow Cytometry

Specifications

• Antigen: SSEA-4
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: MC813-70
• Conjugate: PE-CF594
• Description: Stage-Specific Embryonic Antigen-4
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human Teratocarcinoma Cell Line
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG3 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov