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SSEA-4 Mouse, V450, Clone: MC813-70, BD

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Catalog No. BDB561156 Supplier BD Biosciences Supplier No. 561156
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Description

Mouse Monoclonal Antibody

The MC813-70 monoclonal antibody reacts with Stage-Specific Embryonic Antigen-4 (SSEA-4), a carbohydrate epitope on the major ganglioside, but not the neutral glycolipid, of human teratocarcinoma cells. As its name implies, the expression of SSEA-4 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern differs in the human and mouse. In the human, SSEA-4 is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (ICM), embryonic stem (ES) cells, and the K562 erythromyeloid leukeumia cell line. As human stem cells undergo differentiation, SSEA-4 expression is lost. In the mouse, SSEA-4 is found on oocytes and early cleavage-stage embryos, and primitive ectoderm, but not on EC, ICM, or ES cells. In some cases, SSEA-4 expression appears upon differentiation of mouse EC or ES cells.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the V iolet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

TRUSTED_SUSTAINABILITY

Specifications

Antigen SSEA-4
Applications Flow Cytometry
Classification Monoclonal
Clone MC813-70
Conjugate Violet Fluor 450 (V450)
Description Stage-Specific Embryonic Antigen-4
Formulation Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human Teratocarcinoma Cell Line
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human, Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG3 κ
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