Stat3 (pS727) Mouse, Alexa Fluor 488, Clone: 49/P-STAT3, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 558085

Catalog No. BDB558085

Description

The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6. Stat3 is a 92kDa protein that is activated as a DNA binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 is phophorylated at serine 727 (S727) via the MAPK pathway. The S727 residue is located at a conserved Pro-X-Ser-Pro sequence, which is recognized by the protein kinase ERK. Activation through the S727 residue is thought to lead to initiation of transcription. Upon activation, Stat3 dimerizes, translocates to the nucleus, and binds DNA response elements thereby regulating gene expression. It appears that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to serine phosphorylation, Stat3 is also phosphorylated at tyrosine 705 by JAK1 in response to cytokine stimulation. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-γ-induced genes. Thus, phosphorylation of S727 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity. The 49/p-Stat3 monoclonal antibody recognizes the S727-phosphorylated form of Stat3 (isoform 1). The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (Mfr. No.612542 or 612543) is also effective for western blot analysis of human, mouse, and rat tissue.

Host Species: Mouse
Clone: 49
Isotype: IgG1
Species Reactivity: Human
Immunogen: Phosphorylated Human Stat3

Flow Cytometry, Intracellular Staining

Specifications

• Antigen: Stat3 (pS727)
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 49/P-STAT3
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human Stat3
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Human, Mouse, Rat
• Isotype: IgG1

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.