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Stat3 (pY705) Mouse, Unlabeled, Clone: 4/P-STAT3, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 612356

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Catalog No. BDB612356


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Description

Description

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat3 is a 92kDa protein that is activated as a DNA- binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 activation occurs via tyrosine phosphorylation at Y705. Tyrosine phosphorylation in response to cytokine stimulation is generally mediated by JAK1. Upon activation, Stat3 dimerizes, translocates to the nucleus and binds DNA response elements, thereby regulating gene expression. It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to tyrosine phosphorylation, Stat3 is also phosphorylated at S727 via the MAPK pathway. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-γ induced genes. Thus, phosphorylation of Y705 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity.

Intracellular Staining, Western Blotting

Specifications

Specifications

Stat3 (pY705)
4/P-STAT3
Unconjugated
Affinity Purified
Phosphorylated Human Stat3 Peptide
50μg
Cell Biology
Primary
Human, Murine
Western Blot
250μg/mL
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Murine
IgG2a, κ
RUO
Store undiluted at -20°C.
Monoclonal
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