SV40 Large T and Small T Antigens Mouse, Biotin, Clone: PAB 108, BD

Catalog No.	Quantity
BDB554151	0.1 mg

Catalog No. BDB554151 Supplier BD Biosciences Supplier No. 554151

Description

Mouse Monoclonal Antibody

Simian virus 40 (SV40) is a small DNA virus encoded by 5.2 kb of double-stranded DNA. SV40 large T-antigen (T-ag) is a multifunctional ∽85kDa phosphoprotein, which is the sole viral protein required for SV40 replication. All other factors are provided by the infected host cell. In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines. Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated. The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines. T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter. In addition to transcriptional regulation, T-ag is involved in viral DNA replication. Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis, ATPase, and helicase activities. Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis, and provision of a host-range function for viral replication. However, functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation, and adenylation. T-ag exists in monomeric as well as polymeric forms, and associates with the tumor suppressor proteins p53 and retinoblastoma protein (Rb). Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface. Small t-Ag is a polypeptide which shares 82 N-terminal amino acids with large T antigen and has a unique C-terminal region. Clone PAb 108 recognizes an N-terminal epitope within the first 82 amino acids of T-ag and small t antigen (t-ag). B4 SV40-transformed BALB/c mouse fibroblasts were used as immunogen. PAb 108 was originally produced and characterized as part of a panel of antibodies designated PAb 102-117.

Host Species: Mouse
Clone: PAb 108
Isotype: IgG2a
Species Reactivity: Viral-transformed cells
Immunogen: SV40-transformed BALB/c mouse cells
Formula Weight [Chemical]: 90-100kDa

Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blotting

Specifications

• Antigen: SV40 Large T and Small T Antigens
• Applications: Immunofluorescence
• Classification: Monoclonal
• Clone: PAB 108
• Concentration: 0.5mg/mL
• Conjugate: Biotin
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: SV40-transformed BALB/c mouse cells
• Purification Method: Affinity Purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Virus
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a

For Research Use Only.