MilliporeSigma™ anti-Synapsin-2 Clone: 19.21,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABN1584

Catalog No. MABN1584MI

Description

Specifically detects Synapsin-2 Clone: 19.21 in Human, Mouse, Rat samples, and it is validated for Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting

Synapsin-2 (UniProt Q63537; also known as Synapsin II) is encoded by the Syn2 gene (Gene ID 29179) in rat species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1-2 months of life. The NH2-terminal region is divided in A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The c-terminal regions contain spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn Iia and Iib, domain H in Syn Iia, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.

Specifications

• Antigen: Synapsin-2
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: Q63537
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 19.21 reacts with both synapsin-2 spliced isoforms (IIa and IIb). Clone 19.21 does not react with any of the two synapsin-1 spliced isoforms (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG2a κ

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: 19.21
• Formulation: Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: Syn2
• Immunogen: Purified recombinant rat synapsin-2.
• Quantity: 100 μg
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_001029192
• Target Species: Human, Mouse, Rat
• Form: Purified

For Research Use Only