MilliporeSigma™ anti-Synaptophysin Clone: SP15,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 57382250UL

Catalog No. 57-382-250UL

Description

Specifically detects Synaptophysin Clone: SP15 in Bovine, Feline, Hamster, Human, Mouse, Porcine, Rat samples, and it is validated for Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Immuno Blotting

Synaptophysin is a ∽38kDa integral membrane protein on synaptic vesicles. This protein consists of four transmembrane domains, with its amino- and carboxyl-terminus facing the cytoplasm. Due to its transmembrane domain structure, synaptophysin has been postulated to be a gap junction-like protein as voltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes. Physiological evidence for synaptophysin’s role in secretion includes its involvement in calcium-dependent glutamate secretion in Xenopus oocytes injected with rat cerebellum mRNA.

Specifications

• Antigen: Synaptophysin
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Feline, Hamster, Human, Mouse, Pig, Rat
• Form: Purified

• Applications: Immunoblot, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: SP15
• Formulation: In PBS, 50% glycerol, pH 7.2.
• Immunogen: synaptic vesicle-containing fractions from human brain homogenates
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽38kDa synaptophysin protein in brain tissue. Does not cross-react with the ∽65kDa form in mouse liver extracts.
• Content And Storage: −20°C
• Isotype: IgM

For Research Use Only