Syndecan-4 Rat anti-Mouse, Unlabeled, Clone: KY/8.2, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 550350

Catalog No. BDB550350

Description

The KY/8.2 mAb reacts with mouse syndecan-4, a cell-surface heparan sulfate proteoglycan. Although the ligand(s) for this molecule is unknown, syndecans are known to interact with the heparin-binding domain of growth factors, chemokines, and extracellular matrix components. Accordingly, heparin inhibits the binding of syndecan-4 to the pre-B cell line BC7.12. Syndecan-4 is localized to focal adhesions and binds protein-kinase Cα. Thus, it is involved in cytoskeletal organization and signaling. Syndecan-4 is expressed on stromal cells and B-lymphocyte precursors in bone marrow, in addition to most splenic B cells and a small population of T lymphocytes. It is highly expressed on activated B lymphocytes, but is downregulated in isotype-switched memory B cells. The ectodomain of syndecan-4 is shed from cell surfaces constitutively and in response to specific physiological signals, and the resulting soluble fragments are functional. Immobilized KY/8.2 mAb induced the formation of dendritic processes on syndecan-4-transfected cells and on anti-CD40-activated splenic B cells. Other methods of activating B cells have not produced the same effect. This antibody recognizes an epitope in the N-terminus of syndecan-4, regardless of post-translational modifications.

Host Species: Rat
Clone: KY/8.2
Isotype: IgG2a κ
Species Reactivity: Mouse
Immunogen: BCB10 mouse pre-B cell line

ELISA, Flow Cytometry

Specifications

• Antigen: Syndecan 4
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: BCB10 mouse pre-B cell line
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: KY/8.2
• Conjugate: Unconjugated
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.