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T-bet Mouse, Unlabeled, Clone: O4-46, BD
Mouse Monoclonal Antibody
Supplier: BD Biosciences 561263
Description
The O4-46 monoclonal antibody specifically binds to human and mouse T-bet. T-bet (T-box gene expressed in T cells) is a master regulatory transcription factor that is also known as TBX21 (T-box21) and TBLYM (T-box transcription factor, expressed in lymphocytes). Human (535 amino acids; 58.3kDa predicted molecular mass) and mouse (530 amino acids; 57.7kDa) T-bet proteins are encoded by the human TBX21 (chromosome 17) and mouse Tbx21 (chromosome 11) genes. The human and mouse T-bet protein amino acid sequences are 88% homologous. Human and mouse T-bet proteins share a highly conserved (98% homologous amino acid sequences) T-box protein domain that is centrally located and mediates binding to DNA. T-bet is expressed by and activates transcriptional activities within hemotopoietic cells including stem cells, NK and NKT cells and subsets of thymocytes, primed/activated CD4+ T cells, CD8+ T cells and γδ T cells, B cells, and dendritic cells. Interferon-gamma (IFN-γ), interleukin-27 (IL-27), and IL-12 act on peripheral antigen-triggered (TCR-signaling) T cells to increase T-bet expression. With respect to T helper lymphocytes, T-bet directs the differentiation of naive CD4+ precursor T cells to become Th1-like effector and memory cells. T-bet accomplishes this by activating Th1 genetic programs (including epigenetic modifications) while repressing opposing T helper subset programs. T-bet controls the upregulated expression of the Th1 signature cytokine, IFN-γ, the IL-12Rβ2 subunit and the Runx3 transcription factor and can repress the function of other transcriptional regulators, such as GATA-3 (master regulator of Th2 development) and the expression of other cytokines including IL-2, IL-4 and IL-5. This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488nm), green (532nm) and yellow-green (561nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20nm filter).
Immunohistochemistry (Paraffin), Intracellular Staining, Western Blotting

Specifications
T-bet | |
Monoclonal | |
0.5mg/mL | |
T-box expressed in T cells; TBX21; T-box 21; TBLYM | |
Mouse | |
Affinity Purified | |
RUO | |
Human, Mouse | |
IgG1 κ |
Western Blot | |
O4-46 | |
Unconjugated | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Human T-bet Peptide | |
0.1 mg | |
Primary | |
Store undiluted at 4°C |
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