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Description
NFκB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. In most cells, NF-κB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkBα, IkBβ, and IkBε. Stimulation induces the release, activation, and nuclear translocation of NF-κB. Release of NF-κB results from the phosphorylation and proteolytic degradation of the IκB proteins. Two cytokine-inducible IκB kinases (IKKα and IKKβ) phosphorylate and target the IκB proteins for degradation via the ubiquitin pathway. IKKγ/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-κB downstream of the tumor necrosis factor and Toll-like receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-κB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172 (S172). The J133-1171 monoclonal antibody recognizes the phosphorylated S172 of human TBK1.
Western Blotting
Specifications
Specifications
| Antigen | TBK1 (pS172) |
| Applications | Western Blot |
| Classification | Monoclonal |
| Clone | J133-1171 |
| Concentration | 0.5mg/mL |
| Conjugate | Unconjugated |
| Description | T2K, NAK |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Phosphorylated Human TBK1 |
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