MilliporeSigma™ anti-TCR beta, Clone: H57-597,

Catalog No.	Quantity	Conjugate
MABF931MI	100 μg	Unconjugated

Catalog No. MABF931MI Supplier MilliporeSigma™ Supplier No. MABF931

Description

Armenian Hamster Monoclonal Antibody

Specifically detects TCR β Clone: H57-597 in Mouse samples, and it is validated for Activity Assay, Flow Cytometry, Immunoprecipitation

The T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha (α) and beta (β) dimer, while among 5% of T cells the TCR is composed of a gamma (γ) and a delta (δ) dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The C domain lies proximal to the plasma membrane, while the V domain engages the antigen/MHC complex. Similar to immunoglobulin light chain and heavy chain, the TCR alpha and beta genes (Gene ID 21473 and 21577, respectively, in murine species) undergo VJ and VDJ recombination to generate TCR diversity for recognizing a wide range of antigens. the alpha and beta chains have very short cytoplamic tail and reply on the CD3 (cluster of differentiation 3) co-receptor for transducing signaling further intracellularly.

Specifications

• Antigen: TCR β
• Applications: Functional Assay, Flow Cytometry, Immunoprecipitation
• Classification: Monoclonal
• Clone: H57-597
• Conjugate: Unconjugated
• Formulation: Purified armenian hamster monoclonal IgG antibody in PBS without preservatives.
• Host Species: Armenian Hamster
• Immunogen: Purified TCR from mouse T cells.
• Purification Method: Protein G purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Inflammation, Immunology
• Primary or Secondary: Primary
• Test Specificity: Clone H57-597 targets the C region of TCR beta chain. Clone H57-597 reacts with alpha/beta TCR, but not with gamma/delta TCR, nor with CD3 (Kubo, R.T., et al. (1989). J. Immunol. 142(8):2736-2742).
• Target Species: Mouse
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG

For Research Use Only