TCR αβ Mouse anti-Human, BUV737, Clone: T10B9.1A-31 (also known as T10B9), BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 564725
The T10B9.1A-31 monoclonal antibody specifically binds to a monomorphic determinant on the αβ T-cell receptor expressed on greater than 95% of normal peripheral blood CD3+ T cells. The αβ TCR recognizes a peptide bound to MHC leading to T-cell activation. The T10B9.1A-31 antibody induces T-cell activation in the immobilized form and is useful in flow cytometry for studying αβ + T-cell populations.
The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor 700-like dyes (e.g., 712/20-nm filter).
Due to spectral differences between labeled cells and beads, using BD CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (e.g., CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.
|T10B9.1A-31 (also known as T10B9)|
|αβ TCR; TCR alpha-beta|
|Human Peripheral Blood T Cells|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Brilliant Ultraviolet 737|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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