TCR Beta Chain Hamster anti-Mouse, Brilliant Violet 605, Clone: H57-597, BD

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 562840

Catalog No. BDB562840

Description

The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes and peripheral T lymphocytes and NK1.1+ thymocytes and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before expression of the TCR α-chain. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.

This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

• Antigen: TCR Beta Chain
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Tcrb; TCRbeta; TCRβ, T cell receptor beta chain
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2

• Applications: Flow Cytometry
• Clone: H57-597
• Conjugate: Brilliant Violet 605
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov