TCR Beta Chain Hamster anti-Mouse, PE-CF594, Clone: H57-597, BD

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 562841

Catalog No. BDB562841

Description

The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes and peripheral T lymphocytes and NK1.1+ thymocytes and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before expression of the TCR α-chain. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).

Specifications

• Antigen: TCR Beta Chain
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Tcrb; TCRbeta; TCRβ, T cell receptor beta chain
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2

• Applications: Flow Cytometry
• Clone: H57-597
• Conjugate: PE-CF594
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
• Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov