TCR Beta Hamster anti-Mouse, Brilliant Violet 510, Clone: H57-597, BD

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 563221

Catalog No. BDB563221

Description

The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes and peripheral T lymphocytes and NK1.1+ thymocytes and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before expression of the TCR α-chain. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: TCR Beta
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Tcrb; TCRbeta; TCRβ, T cell receptor beta chain
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2

• Applications: Flow Cytometry
• Clone: H57-597
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.