TFIIB Mouse, Unlabeled, Clone: 24, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 610462

Catalog No. BDB610462

Description

TFIIB is a ubiquitous factor required for transcription initiation by RNA polymerase II. Studies suggest that TFIIB serves as a bridge between the “TATA”-binding factor (TFIID) and RNA polymerase II during pre-initiation complex assembly and that TFIIB can be a target of acidic activators. An essential component of the machinery that transcribes protein-coding genes, the three-dimensional structure of the human TFIIB appears to consist of two direct repeats that adopt similar alpha-helical folds, conferring pseudo-twofold symmetry. An extensive, central basic surface, including an amphipathic alpha helix, is critical to the function of TFIIB as a bridge between the TBP-promoter complex and transcription factors. TFIIB has a calculated molecular weight of 33kDa and has been reported to be observed to migrate between 33-40kDa.

Immunofluorescence, Immunoprecipitation, Western Blotting

Specifications

• Antigen: TFIIB
• Clone: 24
• Conjugate: Unconjugated
• Format: Affinity Purified
• Immunogen: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Quantity: 50μg
• Research Discipline: Cell Biology
• Monoclonal or Polyclonal: Monoclonal

• Applications: Immunofluorescence, Immunoprecipitation, Western Blot
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Host Species: Murine
• Isotype: IgG_2a
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Canine, Frog, Human, Murine, Rat

For Research Use Only.