MilliporeSigma™ anti-Tie2 Clone: Ab33,

Catalog No.	Quantity	Conjugate
61-020-550UG	50 μg	Unconjugated

Catalog No. 61-020-550UG Supplier MilliporeSigma™ Supplier No. 61020550UG

Description

Mouse Monoclonal Antibody

Specifically detects Tie2 Clone: Ab33 in Human, Mouse, Porcine, Rat samples, and it is validated for Immunohistochemistry (Frozen), Immunoprecipitation, Immuno Blotting

Angiopoietin-1 and its naturally occurring inhibitor, angiopoietin-2, exert their effects by inducing autophosphorylation of their signaling receptor Tie2. Phosphorylation of the receptor occurs during vasculogenesis and is required for its activity in regulating endothelial cell proliferation, differentiation and guiding the proper patterning of endothelial cells during blood vessel formation. Tie-2 is a transmembrane receptor-tyrosine kinase that is expressed by endothelial cells and primitive hematopoietic tissues. Tie2/Tek has been shown to be critical for endothelial cell survival, which is evidenced in part by the constitutive phosphorylation that is observed in quiescent endothelium. More recently a multifunction docking site has been identified on the receptor (Tyr¹¹⁰⁰) that serves as a binding site for other partners including Grb2, Grb7, and p85 and acting via an SH2 domain interaction.

Specifications

• Antigen: Tie2
• Applications: Immunoblot, Immunohistochemistry (Frozen), Immunoprecipitation
• Classification: Monoclonal
• Clone: Ab33
• Conjugate: Unconjugated
• Formulation: In 150mM NaCl, 70mM Tris-glycine, 30% glycerol, pH 7.4.
• Host Species: Mouse
• Immunogen: a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His·Tag™ sequence
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽140-45kDa Tie2 protein.
• Target Species: Human, Mouse, Pig, Rat
• Content And Storage: −20°C
• Form: Purified
• Isotype: IgG1

For Research Use Only