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Description
Tumor necrosis factor α (TNF-α) is a macrophage/monocyte cytokine, usually found as a soluble secreted protein, which has diverse immunomodulatory, anti-tumor, and toxic effects.
Host Species: Mouse
Clone: J1D9
Isotype: IgG1
Cross Reactivity: Human; does not react with mouse. Others-not known.
Immunogen: Recombinant human TNF-α
Molecular Weight: 17kDa
Positive Control: TNF-α recombinant protein
Cellular Localization: Cytoplasmic and extracellular
Ab-1 neutralizes the recombinant TNF-alpha mediated cytotoxicity of L929 cells and inhibits the tumor growth in mice. It protects mice against the toxicity of recombinant human TNF-alpha.
Cytotoxicity Assay, ELISA, Flow Cytometry, Western Blotting
Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly
Specifications
Specifications
| Antigen | TNF alpha Ab-1 |
| Classification | Monoclonal |
| Concentration | 200μg/mL |
| Conjugate | Biotin |
| Host Species | Mouse |
| Immunogen | Recombinant human TNF-α |
| Quantity | 1 mL |
| Primary or Secondary | Primary |
| Target Species | Human |
| Isotype | IgG1 |
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