Tomosyn Mouse, Unlabeled, Clone: 15, BD

Catalog No.	Quantity
BDB611296	50 μg

Catalog No. BDB611296 Supplier BD Biosciences Supplier No. 611296

Description

Mouse Monoclonal Antibody

Neuronal signal transmission and neurotransmitter release from the presynaptic nerve terminal is mediated by the synaptic vesicle cycle. Syntaxin plays a central role during vesicle docking and fusion through interactions with many vesicle components. During docking, vSNAREs (VAMP/synaptobrevin, synaptotagmin) on the synaptic vesicle and tSNAREs (SNAP-25, syntaxin) on the plasma membrane interact to form a 7S complex, which is essential to docking and fusion. Syntaxin associates with munc18/n-sec1 prior to and/or during the
formation of the 7S complex. This interaction may inhibit syntaxin binding proteins (VAMP, SNAP-25) that facilitate vesicle docking or fusion. Tomosyn, a syntaxin binding protein, displaces munc18 from syntaxin-1 and forms a novel 10S complex with syntaxin-1, SNAP-25 and synaptogamin. There are two splice variants of tomosyn designated b-tomosyn and s-tomosyn, while the original is referred to as m-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.

Host Species: Mouse
Clone: 15
Isotype: IgG1
Species Reactivity: Rat
Immunogen: Rat Tomosyn aa. 910-1105
Formula Weight [Chemical]: 130kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: Tomosyn
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 15
• Concentration: 250μg/mL
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Rat Tomosyn aa. 910-1105
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Content And Storage: Store undiluted at -20°C.
• Isotype: IgG1