MilliporeSigma™ anti-Topoisomerase II Clone: SWT3D1,

Catalog No.	Quantity	Conjugate
NA14100UG	100 μg	Unconjugated

Catalog No. NA14100UG Supplier MilliporeSigma™ Supplier No. NA14100UG

Description

Mouse Monoclonal Antibody

Specifically detects Topoisomerase II Clone: SWT3D1 in Human samples, and it is validated for Immunohistochemistry (Paraffin)

Topoisomerase II is a nuclear enzyme whose primary function is to induce temporary double-strand breaks in DNA and to facilitate the passage of the duplex DNA segments through these breaks. Phosphorylation of topoisomerase II in a cell cycle regulated manner appears to be important for progression through the cell cycle. The enzyme exists as two discrete isozymes, topoisomerase IIα (∽170kDa) and topoisomerase IIβ (∽180kDa) which are under independent transcriptional regulation. Both isozymes have been identified in human and mouse cells but only the topoisomerase IIα form appears in yeast. The gene encoding topoisomerase IIα has been mapped to chromosome 17 and is in close proximity to the gene for erbB-2. Altered expression of topoisomerase II has been documented in many cancers including AML, CLL, small cell lung cancer, breast and ovarian cancer, and colon cancer. Based on this observation, topoisomerase II has been identified as the target of many widely used antineoplastic drugs as well as the target of rational drug design.

Specifications

• Antigen: Topoisomerase II
• Applications: Immunohistochemistry (Paraffin)
• Classification: Monoclonal
• Clone: SWT3D1
• Conjugate: Unconjugated
• Formulation: In 50mM sodium phosphate buffer, 2% gelatin.
• Host Species: Mouse
• Immunogen: recombinant human topoisomerase IIα
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes ∽170kDa topoisomerase II protein in HeLa cells and breast carcinoma tissue.
• Target Species: Human
• Content And Storage: 2°C to 8°C
• Form: Purified
• Isotype: IgG1

For Research Use Only