Invitrogen™ RNF216 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA141317

Catalog No. 01-675-654

Description

Suggested positive control: human spleen protein, antigen standard for RNF216 (transient overexpression lysate), human lung protein, human ovary protein, human pancreas protein, human skeletal muscle protein, human heart protein, human kidney protein, human liver protein, human brain protein, human brain tissue, heart tissue, kidney tissue, liver tissue, lung tissue, ovary tissue, pancreas tissue, small intestine tissue, skeletal muscle tissue, spleen tissue, human small intestine protein.

Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.

Specifications

• Antigen: RNF216
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RNF216
• Gene Alias: 2810055G22Rik; AI647468; AU019462; C86502; CAHH; E3 ubiquitin ligase; E3 ubiquitin-protein ligase RNF216; F830018F18Rik; RGD1565135; RING finger protein 216; RING-type E3 ubiquitin transferase RNF216; RNF216; Triad domain-containing protein 3; TRIAD3; triad3a; U7I1; UBCE7IP1; UbcM4-interacting protein 83; ubiquitin-conjugating enzyme 7-interacting protein 1; Uip83; Zin; zinc finger protein inhibiting NF-kappa-B
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 54476
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.2% gelatin and 0.05% sodium azide
• Gene Accession No.: Q9NWF9
• Gene Symbols: RNF216
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of human TRIAD3 between amino acids 350-400.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG