MilliporeSigma™ anti- TRIM3 Clone: 13D12.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC945

Catalog No. MABC945MI

Description

Specifically detects TRIM3 Clone: 13D12.1 in Human, Mouse, Rat samples, and it is validated for Immunohistochemistry, Western Blotting

Tripartite motif-containing protein 3 (UniProt O75382; also known as Brain-expressed RING finger protein, RING finger protein 22, Ring finger protein 97, Tripartite motif protein TRIM3, Tripartite motif-containing 3) is encoded by the TRIM3 (also known as BERP, HAC1, RNF22, RNF97) gene (Gene ID 10612) in human. TRIM3 belongs to a family of more than 70 TRIM/RBCC proteins characterized by their shared tripartite motif structure, also known as the RBCC (RING finger, B-box, and coiled-coil) domain. The B-box and coiled coil regions are believed to participate in protein-protein interactions and formation of macromolecular complexes. TRIM proteins are involved in diverse cellular processes, including cell proliferation, differentiation, oncogenesis, and apoptosis. The presence of a RING domain suggests that TRIM proteins function as E3 ubiquitin ligases. TRIM3 is reported to catalyze the transfer of ubiquitin from UbcH5a to p21, a protein necessary for the proliferation of a subset of PDGF (platelet-derived growth factor)-transformed proneural glioma cells. Loss of TRIM3 heterozygosity is found in more than 10% of human GBMs (glioblastomas), while reducing TRIM3 expression facilitates tumor development and accelerates the progression of oligodendroglioma in a mouse model.

Specifications

• Antigen: TRIM3
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: O75382
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Expected to react with all four spliced isoforms.
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG1 κ

• Applications: Immunohistochemistry, Western Blot
• Clone: 13D12.1
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: TRIM3, BERP, RNF22, RNF97
• Immunogen: GST-tagged recombinant protein corresponding to the internal fragment of human TRIM3.
• Quantity: 100 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_001234935
• Target Species: Human, Mouse, Rat
• Form: Purified

For Research Use Only