MilliporeSigma™ anti-uPA Clone: 16,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ IM15L100UG

Catalog No. IM15L100UG

Description

Specifically detects uPA Clone: 16 in Human samples, and it is validated for Immunoprecipitation

Urokinase-type plasminogen activator (uPA) is a serine proteinase that functions in the conversion of plasminogen to the active proteinase, plasmin1. It is secreted from cells as an inactive 52kDa precursor (single-chain or sc-uPA) which is activated to the two-chain form consisting of an A and B-chain. The active high molecular weight form (HMW-uPA) can be further processed by removal of an amino-terminal fragment (ATF) to an active low molecular weight form (LMW-uPA) of ∽33kDa. The B-chain contains the active site and binds the two endogenous plasminogen activator inhibitors, PAI-1 and PAI-22. The binding of pro-uPA to its cell surface receptor enhances its rate of activation3. uPA is responsible for normal physiological processes such as tissue remodeling and cell migration. Since cell surface plasminogen activation is involved in extracellular matrix degradation, uPA is likely to play a key role in the process of invasion and metastasis.

Specifications

• Antigen: uPA
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

• Applications: Immunoprecipitation
• Clone: 16
• Formulation: Lyophilized from 20mM NH₄HCO₃, 100μg BSA.
• Immunogen: the B chain of human urokinase plasminogen activator
• Regulatory Status: RUO
• Test Specificity: Recognizes uPA/PAI complexes in dexamethasone-treated HT-1080 cells and carcinoma tissue. Sold under license of U.S. Patent 5,422,245.
• Content And Storage: 2°C to 8°C
• Isotype: IgG1

For Research Use Only