Villin Mouse anti-Human, Unlabeled, Clone: 12, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 610358

Catalog No. BDB610358

Description

The isolated intestinal microvillus cytoskeleton (core) consists of four major proteins: actin, villin, fimbrin, and brush border myosin-I. These proteins can assemble in vitro into structures resembling native microvillus cores. Of these components, villin, and brush border myosin-I show tissue-specific expression, so they may be involved in the morphogenesis of intestinal microvilli. Found in association with the microvillar actin bundles of the intestinal brush border, villin is a 95kDa protein composed of two very similar domains of approximately 44kDa each, the core, and a C-terminal domain of 8kDa, the headpiece. The core has been shown to contain villin's Ca²⁺ regulated capping, nucleating, and severing activities, but it cannot induce the formation of microfilament bundles without the headpiece. Villin is a useful differentiation marker of early embryogenesis and may be useful in diagnosis and follow-up of colorectal cancers. It has been demonstrated that villin is necessary for both the cytoskeletal and membrane protein organization of a functional brush border.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: Villin
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1

• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Clone: 12
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.