Vti1a Mouse, Unlabeled, Clone: 45, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 611220

Catalog No. BDB611220

Description

Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. VTI1a and VTI1b are putative mammalian SNARE proteins identified by sequence comparison with yeast SNAREs. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. Vti1a, a possible t-SNARE, contains a C-terminal hydrophobic domain and several regions that may form coiled-coil structures. It exists in distinct syntaxin 5- and syntaxin 6-containing SNARE complexes within the Golgi apparatus. Inhibition of Vti1a blocks transport of G proteins to the cell surface and results in their accumulation within the Golgi. Thus, Vti1a functions in protein transport within the secretory pathway.

Host Species: Mouse
Clone: 45
Isotype: IgG1
Species Reactivity: Rat
Immunogen: Mouse Vti1a aa.114-217
Formula Weight [Chemical]: 29kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: Vti1a
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Mouse Vti1a aa.114-217
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Canine, Human, Mouse, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 45
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov