Invitrogen™ APAF1 Monoclonal Antibody (2H9A1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA548371

Catalog No. FERMA548371

Description

APAF1 Monoclonal Antibody for Western Blot, ICC/IF, Flow, ELISA

APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Specifications

• Antigen: APAF1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: O14727
• Gene Symbols: APAF1
• Immunogen: Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: 2H9A1
• Conjugate: Unconjugated
• Gene: APAF1
• Gene Alias: 6230400I06Rik; APAF1; APAF-1; Apaf1l; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; apoptotic protease-activating factor 1; CED4; DKFZp781B1145; fog; forebrain overgrowth; KIAA0413; mKIAA0413
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 317
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid