Invitrogen™ APC Monoclonal Antibody (eBioAPC-6A2), Biotin, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 13417082

Catalog No. 13-417-082

Description

Description: The eBioAPC-6A2 monoclonal antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using eBioAPC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. The eBioAPC-6A2 can be used for separation of cells labeled with APC-conjugated antibodies or for staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary APC-conjugated antibody and the application tested. Applications Reported: This eBioAPC-6A2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioAPC-6A2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained with an APC-conjugated antibody. This can be used at less than or equal to 0.015 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Anti-APC (Allophycocyanin) antibodies can be used to bind APC-conjugated primary antibodies without decreasing fluorescent intensity of the APC fluorochrome. This method is used for separation of cells labeled with APC-conjugated antibodies.

Specifications

• Antigen: APC
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid
• Target Molecule: Tag

• Applications: Flow Cytometry
• Clone: eBioAPC-6A2
• Conjugate: Biotin
• Gene Alias: APC
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG1

Safety and Handling

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